AMPK/FOXO3a Pathway Increases Activity and/or Expression of ATM, DNA-PKcs, Src, EGFR, PDK1, and SOD2 and Induces Radioresistance under Nutrient Starvation

Most solid tumors contain hypoxic and nutrient-deprived microenvironments. The cancer cells in these microenvironments have been reported to exhibit radioresistance. We previously that nutrient starvation increases the expression and/or activity of ATM DNA-PKcs, which are involved repair DNA double-strand breaks induced by ionizing radiation. In present study, elucidate molecular mechanisms underlying phenomena, we investigated roles AMPK FOXO3a, play key cellular response starvation. Nutrient increased clonogenic cell survival after irradiation AMPKα, ATM, Src, EGFR, PDK1, SOD2 MDA-MB-231 cells. Knockdown AMPKα using siRNA suppressed under FOXO3a decreased incidence apoptosis 8 Gy irradiation. appear be molecules induce radioresistance may serve as targets for radiosensitization.